Alcohol-preserved sub-samples from BSS MOC nets #2,3,4 (150 µm)

This protocol is included as a standard procedure on all Broad-scale cruises. The goal is to obtain portions of the stratified samples of Pseudocalanus spp. for the 1 m2 MOCNESS 150 µm nets for preservation in ethanol for molecular analysis.

Protocol for Pseudocalanus spp. collection during Broad-scale cruises

  1. Stations to be sampled: 3, 4, 7, 9, 12, 13, 16, 17, 18, 20, 27, 29, 30, 36, and 38 (This list includes all 1st priority and some 2nd priority stations). Nets to be sampled: #2, #3, #4.

PROTOCOL

  1. After sample is rinsed from cod end to bucket, collect on 150 µm screen and rinse into a 1 liter beaker bringing up volume to equal 1 liter (or 600 mls, or 800 mls depending on how concentrated the sample is);

  2. Swirl to homogenize the sample as much as possible;

  3. Remove subsample with scoop (90 mls) and pour into small diameter screen (150 µm mesh);

  4. Rinse the drained plankton with 95% ethanol into a 4 oz. glass jar;

  5. Add 95% ethanol - making sure that there is 3 to 4 times more alcohol than plankton volume;

  6. Change alcohol after 24 hours;

  7. Record volume removed on preservation sheet (i.e. 90 mls/1000 mls to Ann Bucklin);

  8. Preserve the remainder of the plankton in 10% formaldehyde using the standard protocol and record on preservation sheet if any other subsamples are removed;

  9. Leave Ann Bucklin's samples in Peter Wiebe's lab at WHOI or call Ann to arrange pick-up.

Prepared 1996/04/06 by:
Ann C Bucklin

Ocean Process Analysis Laboratory
Institute for the Study of Earth, Oceans, and Space
142 Morse Hall
University of New Hampshire
Durham, NH 03824-3525

(603) 862-3505
FAX (603) 862-0243