Jason Beaudet, Ann Bucklin,
and Karen Carleton (in prep.)
ØReal-time quantitative PCR (QPCR) allows estimation of
relative abundances of species in unsorted samples.
ØQPCR results for amplification of serially diluted chimeric PCR
templates, with threshhold cycle number, Ct =
0.100 Relative Fluorescent Units (RFU). The chimeric template ensured a 1:1 ratio
between DNA copy numbers for the two species.
ØStandard curves of log copy number (DNA concentration) versus Ct for Pseudocalanus spp. PCR efficiencies for
primers calculated from slopes.
Cryptic Species
Identification and Quantification using QPCR