§Pseudocalanus moultoni and P. newmani are morphologically cryptic but genetically distinct, differing by 18% in mtCOI sequence.
§Multiplexed, species-specific PCR (SS-PCR) was used to identify individual copepods. Reactions were done in 96-well plates, with controls.
§For the U.S. GLOBEC samples, only adult females were identified.
From Bucklin et al. (1999, 2001)
SS-PCR discrimination of cryptic Pseudocalanus spp.
When it’s working: Pickled Copepod Research, Poloroid Captured Ratios
When it’s not working: Pathetically Crappy Results, Personal Contamination of Reagents