Protocol for Pseudocalanus collection
We would like to have splits of the MOCNESS samples from nets
2, 3, and 4 (i.e., the 150 um mesh nets during the uphaul) from all of
the ten PRIORITY 1 stations (BSS #3, 7, 9, 12, 16, 18, 20, 29, 36, and
38) and - if possible - five PRIORITY 2 stations (BSS #4, 13, 17, 27, 30).
The protocol would be the same as is currently done for Charlie Miller,
which is to scoop an approximate 90 ml sub-sample from the stirred
sample. This sub-sample will then be drained over nitex and preserved in
alcohol. We will request this at all of Charlie's stations, which I
don't know yet. The goal is to obtain samples - preserved in alcohol for
molecular analysis - of 100 or so individuals of all juvenile (CI - CV)
and the adult stages of Pseudocalanus spp. at the broad-scale
stations.
Procedure
- Bring the sample volume up to 2 liters in seawater;
- Swirl strongly to homogenize the sample as much as possible;
- Use Miller's scoop to remove approximately 90 ml of sample into a
funnel screened with 150 um mesh nitex;
- Wash the plankton from the net using alcohol into a 4 oz glass jar;
- Add 95% ethanol - making sure that there is 3 to 4X more alcohol
than plankton volume;
- Change alcohol after 24 hrs if possible; and
- Leave samples in Peter Wiebe's lab at WHOI or call Ann Bucklin to
arrange pick-up.
Ann C Bucklin (acb@kepler.unh.edu) and
Sara Franzen (sfranzen@christa.unh.edu)
Created: March 6, 1996