Protocol for Pseudocalanus collection

We would like to have splits of the MOCNESS samples from nets 2, 3, and 4 (i.e., the 150 um mesh nets during the uphaul) from all of the ten PRIORITY 1 stations (BSS #3, 7, 9, 12, 16, 18, 20, 29, 36, and 38) and - if possible - five PRIORITY 2 stations (BSS #4, 13, 17, 27, 30).

The protocol would be the same as is currently done for Charlie Miller, which is to scoop an approximate 90 ml sub-sample from the stirred sample. This sub-sample will then be drained over nitex and preserved in alcohol. We will request this at all of Charlie's stations, which I don't know yet. The goal is to obtain samples - preserved in alcohol for molecular analysis - of 100 or so individuals of all juvenile (CI - CV) and the adult stages of Pseudocalanus spp. at the broad-scale stations.


  1. Bring the sample volume up to 2 liters in seawater;
  2. Swirl strongly to homogenize the sample as much as possible;
  3. Use Miller's scoop to remove approximately 90 ml of sample into a funnel screened with 150 um mesh nitex;
  4. Wash the plankton from the net using alcohol into a 4 oz glass jar;
  5. Add 95% ethanol - making sure that there is 3 to 4X more alcohol than plankton volume;
  6. Change alcohol after 24 hrs if possible; and
  7. Leave samples in Peter Wiebe's lab at WHOI or call Ann Bucklin to arrange pick-up.

Ann C Bucklin ( and Sara Franzen (
Created: March 6, 1996