Zooplankton Abundance Based on Taxa and Life Stages or Size

Collected during the Process Cruises, Austral Autumn 2001 and 2002

Fieldname Description Units
cruiseid Cruise identifier (LMG0104, LMG0203)  
station Station number  
month_local Month of year, local time  
day_local Day of month, local time  
time_local Time local, GMT = local time +4 hours HHmm
event Event number from cruise event log; unique ID  
lat Latitude at beginning of net tow, negative = South decimal degrees
lon Longitude at beginning of net tow, negative = West decimal degrees
tow Net tow number  
net Net number (1-8)  
vol_filt Volume of water filtered by net meters3
displ_vol Displacement volume of sample milliliters
depth_open Depth each net was opened meters
depth_close Depth each net was closed meters
taxon_group There are 3 groups: Copepoda, Euphausiacea or Other Zooplankton  
taxon Scientific name of organism or common name of group  
stage Life history stage, e.g.:
CV = copepodite stage V,
C2 = calyptopis stage 2,
F2 = furcilia stage 2
lt_15mm = size class
 
samp_fraction_denom The subsample split fraction, expressed as the denominator of the given fraction (i.e. 1/16 = 16, 1/400 = 400), a flag value of 6.6667 represents the fraction 3/20 (20/3 = 6.6667)  
count Number of individuals counted in sample fraction  
abundance Number of individuals per cubic meter meters3

Data Contributed By:
Kendra L. Daly
College of Marine Science
University of South Florida
140 Seventh Ave. S.
St. Petersburg, FL 33701

Phone: 727-553-1041
E-Mail: kdaly@marine.usf.edu

Net sample collections: Meng Zhou, Yiwu Zhu, Ryan Dorland, Dan Mertes, and Joe Smith

Sample Analyses: Jason Zimmerman, Alexander Timonin, Tatjana Semenova

Cruises:
Laurence M. Gould, 23 April - 6 June 2001 (LMG0104 cruise report), 18 net tows
Laurence M Gould, 7 April - 21 May 2002 (LMG0203 cruise report), 16 net tows

Methods

Zooplankton abundance, vertical and horizontal distribution, and population structure were assessed using a 1 m2 Multiple Opening and Closing Nets and Environmental Sampling System (MOCNESS) with nine nets having 333 µm mesh and environmental sensors of temperature, salinity and depth.  The entire water column was sampled on the downcast using net # 0, which was not analyzed.  The upper water column was typically sampled using nets 1 and 2 from 500 m to 200 m at 150 m depth intervals, nets 3 and 4 from 200 m to 100 m at 50 m depth intervals, and nets 5 to 8 from 100 m to the surface at 25 m depth intervals.  Some tows were deployed down to 1000 m, and some tows were towed targeting krill swarms at irregular depths.  Approximate locations of process study stations are shown in Fig. 1. More precise locations of the casts in Fig. 2.

Zooplankton samples were immediately preserved in 10% buffered formalin solution.  All the large organisms (>15 mm) in the sample were removed and identified to taxa.  The sample was then split to about 100 individuals of euphausiids.  All euphausiids in the split were identified to species and life history stage and measured for length to the nearest 0.5 mm for larvae and to the nearest 1.0 mm for juvenile and adults.  Next the sample was split to about 100 individuals of copepods.  Copepods were identified to species and life history stage (female, male, copepodite V, or other copepodite). All other zooplankton in the split were identified to taxa and counted.  For the Euchaetidae, we followed the designation of Park (1994) who ascribed the Antarctic species to the genus Paraeuchaeta.  This data object ("krill") reports the abundance of each euphausiid species by life stage and size class. The companion data object "zooabund" reports the counts of zooplankton per subsample/split by taxa and life stage or size class.

Greenwich Mean Time was local time + 4 hours.

Reference:

Park, T. 1994. Taxonomy and distribution of the marine calanoid copepod family Euchaetidae. Bulletin of the Scripps Institution of Oceanography University of California, San Diego v. 29, University of California Press, Berkeley, CA, 203 pp.

Fig. 1. Approximate locations of process cruise stations in Marguerite Bay during austral autumn 2001 and 2002.

MOCNESS station map

Fig. 2. Locations of MOCNESS stations for LMGould-0104 (2001) and LMGould-0203 (2002) cruises. Numbers correspond to the cast number.

Notes on Volume Filtered values: 

The 1 m2 MOCNESS net volume filtered data were corrected for the following net tows:

The flowmeter used to calculate the volume of water filtered did not work for the tows shown below; the data shown in the listings for those tows are therefore derived, not measured. Using the net distance and the average angle of the net, the volume filtered was estimated. These estimates have about a 10% error or higher when currents were present.

LMG0104: St. 4, Cast 9, event #98

Net Volume Filtered (m3)
1 548
2 793
3 490
4 460
5 314
6 276
7 262
8 329

LMG0104: St. 3, Cast 16, event #200

Net Volume Filtered (m3)
1 801
2 789
3 633
4 286
5 280
6 244
7 262

2001    No further corrections.  MOC-1 net volume filtered values were used to calculate abundance/m3 for all net tows.  No Optical Plankton Counter (OPC) volume filtered values were available for 2001.

2002    The flowmeter data was not accurate for some net tows in 2002. Optical Plankton Counter (OPC) volume filtered was used to calculate abundance/m3 for all MOC-1 net tows, except casts 11 and 16 which used the MOC-1 volume filtered values.  The decision to use OPC values was determined by plotting all OPC volume filtered numbers versus MOC-1 flow meter data (see below).  MOC-1 flow meter data were consistently lower and had a lower R2 regression for volume filtered versus length of tow (time).  Meng Zhou and Yiwi Zhu, who conducted the MOC-1 net tows, noted on the haul sheets that there were problems with the MOC-1 flow meters and that they often were not responsive or worked intermittently. Thus, it was decided that the OPC flow meter data provided a more consistent measure of net flow volume for 2002 net tows.  The OPC mouth opening for flow meter data was corrected to the MOC-1 net mouth opening by multiplying the OPC flow data by 200.  The two casts that used MOC-1 flow meter data were cases where the OPC flow meter data appeared to underestimate the flow. 

LMG0202 volume filtered: MOC and OPC

Fig. 3. Volume filtered over time for both MOCNESS and Optical Plankton Counter showing consistently lower flow rates for the MOCNESS.

Notes on missing samples:

LMG0104 - Station 1A, tow 1, net 2: non-quatitative sample
LMG0104 - Station 1A, tow 1, net 8: no sample

Updated October 8, 2004; G. Heimerdinger
Updated July 9, 2008; N. Copley