Now moving on to the population genetics partÉ. In this study, nuclear protein coding genes have been used as population genetic markers. As a brief introduction, the nuclear genes are diploid, which means they occur in 2 copies in the nuclear genome. Here is an example of a chromatogram, Double peaks are read as heterozygotes, while single peaks are homozygotes. Heterozyg sites are the sites at which the 2 copies have different nucleotide bases, which appear superimposed in chromatograms
In this study, 3 metabolic genes have been used for analysis: CS, hsp70 and AMPK.