PowerPoint Presentation

Nuclear protein-coding genes

as population genetic markers

q Diploid genes - occur in two copies in the nuclear genome.

q Double-peaks are read as heterozygous (i.e., sites at which the two copies have different nucleotide bases).

q 3 metabolic genes to be used:

1. Citrate synthase (CS) - metabolic

enzyme of the citric acid cycle

2. Heat shock protein 70 (Hsp70) – molecular

chaperones which stabilize existing proteins against damage

3. AMP-Activated Protein Kinase (AMPK) – cellular energy homeostasis


	Now moving on to the population genetics part�. In this study, nuclear protein coding genes have been used as population genetic markers. As a brief introduction, the nuclear genes are diploid, which means they occur in 2 copies in the nuclear genome. Here is an example of a chromatogram, Double peaks are read as heterozygotes, while single peaks are homozygotes. Heterozyg sites are the sites at which the 2 copies have different nucleotide bases, which appear superimposed in chromatograms
	In this study, 3 metabolic genes have been used for analysis: CS, hsp70 and AMPK.